Bovine Casomorphins Transferring to Babies via Human Milk

Yet another paper from Poland, this one published in the peer-reviewed international Journal of Pediatric Gastroenterology and Nutrition,(1) is providing insights about bovine (cow) casomorphins finding their way into the blood of babies via human milk. This time the casomorphin is BCM5, which is derived from the breakdown of BCM7, which in turn is derived from A1 beta-casein. Until now I have largely ignored bovine BCM5, and in my book ‘Devil in the Milk” I only mentioned it once (p38). (2) But I can see that in future I am going to have to look at it much more closely.

Those of us that are interested in casomorphins and human health have known for a long time that bovine BCM5 is a more powerful opioid than bovine BCM7. One paper makes the comparison that whereas bovine BCM7 is similar in opioid strength to morphine, bovine BCM5 is ten times more powerful than either.(3) However, the accepted evidence has been that it is probably not released by digestion of cow milk in the human gut, whereas BCM7 was definitely released.(4, 5)

What the Polish work is indicating is that even if BCM5 is not normally found in the human gut (and there is a logic why that might be so, related to the DPP4 enzyme that is needed to break down BCM7 to BCM5 only being found within the gut on mesenteric cells, rather than circulating freely in the gut), BCM5 is getting formed from BCM7 in the sera (blood) (where DPP4 does circulate freely) or other tissues. From there it is getting transferred into human milk.  From there it gets into the stomachs and blood of babies.

[Update August 2016: On re-reading this post I see that some material above (which I have now struck out) was incorrect.  Although DPP4 does break down BCM7, it does this by fracturing the peptide at the N terminus between the second and third amino acids of the peptide. The enzyme previously identified in the literature as being capable of breaking down BCM7 to BCM5 is carboxypeptidase Y which acts on the carboxyl end of the peptide.  This correction has no implications for the rest of the post]

If all of this is true – and that is what this latest research is indicating – then the implications are huge.

So what did this Polish research actually report?

The study is reported as a case study of a child suffering bouts of apnoea immediately after breast feeding. But there is much more to it than that, and it is really a case-control with 10 normal babies as the controls.  I am actually more interested in the findings from the controls, but first the case itself.

The mother self diagnosed that the baby was suffering from the apnoea on those occasions when she (the mother) had been drinking large quantities of cow milk. Sure enough, on testing the baby’s blood, extremely high levels of bovine BCM5 were found. (For those that think quantitatively, 15,147 ng/mL.). Other possible causes of the apnoea were investigated but proven negative. So this in itself does not prove causation. But the association (which in science is not quite the same thing as ‘proof’) is a remarkable finding and points in only one direction.

So how does this level actually compare to the levels found in the normal babies?  In these babies it was much lower, ranging from 1 ng to 864 ng/mL. Accordingly, there is a remarkable contrast between the controls and the case, with the case being an extreme outlier.

However, my interest is in the fact that for seven out of eight of the controls (data for the other two is not presented, which is frustrating), considerable levels of BCM5 were found. For the eighth control which had only 1 ng/mL, the mother did not like cow milk and did not drink it, whereas all of the other mothers did. These babies were aged 7-14 weeks and all were exclusively breast-fed.  To reiterate, although much lower than for the case, the levels in these controls are still high.

So here we have evidence of considerable levels of BCM5 getting into babies blood via their mothers’ milk.  This is a remarkable finding, although highly consistent with other work of this group that showed BCM7 was getting through to babies via their mothers’ milk.(6)

Once again we can be confident that the mainstream dairy industry will denigrate this research. They will probably point out that it is only a case study (although actually it is a case-control). And they will point out that the authors themselves emphasise that further work is needed including measuring the BCM5 in the milk of the mothers, and taking blood readings at different times relative to feeding times.

However, what this research does do is throw light into a number of previously dark places.  BCM5 is now looking more important than previously, and this paper does provide confirmatory evidence that nasty peptides can get from cows’ milk to human babies via their mother’s’ milk.  What Mum eats and drinks is important for baby.  If Mum drinks milk containing A1 beta-casein, then it would seem that baby is gong to drink casomorphins.

REFERENCES

1.  Wasilewska J, Kaczmarski M, Kostyra E, Iwan M. Cow’s-milk-induced Infant Apnoea With Increased Serum Content of Bovine beta-Casomorphin-5. J Pediatric Gastroenterology and Nutrition. 2011 June; 52(6): 772-775

2.  Woodford K. Devil in the Milk: Illness, Health and Politics: A1 and A2 Milk. Wellington New Zealand: Craig Potton Publishing 2007.

3.  Hedner J, Hedner T. beta-Casomorphins induce apnea and irregular breathing in adult rats and newborn rabbits. Life Sci. 1987 Nov 16;41(20):2303-12.

4.  De Noni I. Release of b-casomorphins 5 and 7 during simulated gastro-intestinal digestion of bovine b-casein variants and milk-based infant formulas. Food Chemistry 2008 110(4):897-903.

5.  Svedberg J, de Haas J, Leimenstoll G, Paul F, Teschemacher H. Demonstration of beta-casomorphin immunoreactive materials in in vitro digests of bovine milk and in small intestine contents after bovine milk ingestion in adult humans. Peptides. 1985 Sep-Oct;6(5):825-30.

6.  Wasilewska J, Sienkiewicz-Szlapka E, Kuzbida E, Jarmolowska B, Kaczmarski M, Kostyra E. The exogenous opioid peptides and DPPIV serum activity in infants with apnoea expressed as apparent life threatening events (ALTE). Neuropeptides. 2011 Jun;45(3):189-95.

About Keith Woodford

Keith Woodford is an independent consultant, based in New Zealand, who works internationally on agri-food systems and rural development projects. He holds honorary positions as Professor of Agri-Food Systems at Lincoln University, New Zealand, and as Senior Research Fellow at the Contemporary China Research Centre at Victoria University, Wellington.
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19 Responses to Bovine Casomorphins Transferring to Babies via Human Milk

  1. Pingback: Around the Web; Back from Vacation Edition | Perfect Health Diet

  2. Warwick Chapman says:

    Having read Your life in your hands by Jane Plant years ago and wondering why milk is one possible factor responsible for breast cancer ,theBCM7 and especially BMC5 could be implicated. My guess is that oxidation damage to DNA being a likely cause. Could the human body have some mechanism of shunting proteins or parts thereof into areas of the body where they will be required, not just immediately but at some future date, in this case breast tissue causing overtime a cancer the immune system when weakened cannot control. Why couldnt the same process start in utero with implications for sensitivities and diseases throughout life. Could these same or similar (slightly changed ) proteins be responsible dementia by locking on to receptors ,oxidizing over time sensitive brain tissue, with resultant lesions, tangles,plaques etc

  3. I have a question in my mind. how A1 cow can be converted into A 2 cow. pl give me step by step procedure to be followed by any one who want to do this. because in India govt. had and is converting large number of A 2 cow in A 1 cow by artificial inseamation of H/F. bulls . why I donot know.

    • Keith Woodford says:

      The conversion from A2 to A1 in India is occurring by accident, because people there do not realise that the A2 version of the beta-casein gene is very common in the ‘improved’ breeds of European origin. The only way to convert to A2 is by genetic testing of animals. In a technical sense this is easy, but I do not know if anyone offers this test in India. Most genetics departments at universities would have the skills to do the test but it is unlikely that they are currently offering it. Most of the big international semen companies do know the status of their bulls and can supply A2 semen if asked (although they do not advertise this widely). But once again, I know nothing about the semen companies in india.
      KeithW

  4. in India.A 2 semen is available.what step I have to take.how much time it will take. how many generation we have to wait to convert A 1 cow into A 2 cow. pl. give us step by step details. further whether you people can advice govt. of India about bad effect of A 1 cows , so that they can stop this wrong practice in India.

    • Keith Woodford says:

      Each cow has two copies of the beta casein gene. To produce beta casein that is 100% A2 then a cow needs two copies of the A2 form of the gene.
      A cow that has one copy of A1 and 1 copy of A2 will produce milk that is half A1 beta casein and half A2 beta casein. This means that if you mate a cow that is A1A2 with semen from a bull that is A2A2 there is 50% chance of getting a pure A2A2 calf and 50% chance of getting a calf that is itself A1A2. The only way to know if the calf is A2A2 is to do a genetic test.
      If the cow is A1A1 and is mated with semen that is from an A2A2 bull, then the calf will be A1A2.
      I have seen some media reports that there is a cattle research station in India that is interested in the A2 issue. I do not recall the details but you may be able to find it by an internet search.
      The issue of A1 and A2 is important for India, becuase all of your native cattle and buffaloes are A2, and this research station isn aware of that.
      But I doubt whether I would have much influence with your Government.
      Keith W

  5. sheo narain beria says:

    how many generation i have to repeat this process ,so that i can get a2a2 cow with 100% surety.pl.give me step by step procedure.pl.give ph value in milk,cow dung,urine, in a1 cow and a2 cow seperetaly.pl. give us the length of intestine of a1 and a2 cow seperately.whethether a1 cow has leaky gut but a2 cow donot have leaky gut.

    • Dinesh Tewatia says:

      I believe at least 7 generations would be required to transform A1 cow to A2 cow by successful breeding with A2 bull.

  6. Ariana says:

    This is interesting. My 9 month old daughter has milk allergies. She has thrown up and broken out in hives on consuming yogurt. Ofcourse I gave her organic milk yogurt but this A1/A2 makes sense now. she couldnt even tolerate Goats milk. I do not consume a lot of dairy but when I do she does have bouts of eczema. I am breast feeding her,which she is fine with.
    I hope she outgrows her allergies. Thank you so much.
    Ariana
    USA

  7. Arnab Guha says:

    Does human breast milk has inherent BCM7 & BCM5 if the lactating mother is not on bovine milk for atleast 9 months?

    • Keith Woodford says:

      Arnab
      We need to distinguish between bovine BCM7 and human BCM7. They have different structures and different pharmacological properties. Production of some human BCM7 (hBCM7) is natural.
      Bovine BCM7 (bBCM7) will only be present in human milk if the mother has been drinking bovine milk in the previous few days.
      We still have need to learn more about BCM5, be that bovine or human. However, BCM5 will be produced in much smaller quantities than BCM7 (for reasons that are well understood but are somewhat complex without going into protein chemistry in some depth). Accordingly, in terms of health implications, my focus is essentially on BCM7 rather than BCM5.
      KeithW

      • Dinesh Tewatia says:

        Hello Keith, does whey protein powder, available in market, containing Histidine as an Essential Amino Acid also form BCM7 while digestion??

      • Keith Woodford says:

        Dinesh
        Whey powder will not produce any BCM7 assuming there is no casein in it.
        However, I do not advocate whey powders.
        There are good reasons why all animals produce milk that contains both casein proteins and whey proteins.
        One of the reasons for beta-casein is that it stops the whey from agglomerating in the gut system.
        The science around these and related issues needed to be further developed. But my starting point is given that nature has determined that all mammal species should produce casein as well as whey then there is likely to be a very good reason for it. At some point, evolutionary mutations for milk that lacks casein will have occurred, but they have never survived. And therein lies a warning.
        Whey proteins digest more quickly than casein and hence a combination of whey and casein gives less of a ‘blood spike’. But I doubt if that is the whole story as to why milks always contain both casein and whey.
        KeithW

      • Arnab Guha says:

        Dear Keith,

        Thanks for your response. It’s great have scientist like you around us and charting out the evolutionary science for mankind.

        We are working on GFCFSF Diet for kids with autism.
        As per https://www.intechopen.com/books/autism-paradigms-recent-research-and-clinical-applications/impact-of-infant-feeding-methods-on-the-development-of-autism-spectrum-disorder#tab2, it stated-
        “children who were breastfed longer than 12 months are 6.67 times less likely to have autism diagnosis than children who were breastfed less than 12 months. Breastfeeding of less than 6 months duration was significantly associated with autism diagnosis.”

        My interpretation are:
        * native hBCM7 has beneficial effects in human & bBCM7 (from A1 bovine milk) which get passed to babies through mother’s milk maybe not good for the babies.
        * critical mass of hBCM7 is important (atleast 12 months of breast feeding vs 6-months)
        * increased gut permeability in leaky gut condition enhances probability of bBCM7 to reach to the brain of a kid with autism.
        * examining the impact of bBCM7 & hBCM7 as well as their counterparts BCM9 in kids with autism will give us faster insights on mechanistic understanding rather than studies mainly on general population.
        * we also need to have better understanding of BCM7 et al. on gut neurotransmitters like Serotonin etc & thymus gland secretions post 8-years of age.

        Please share your thoughts the above statements.

        Thanks & Regards
        Arnab Guha

      • Keith Woodford says:

        Arnab
        I agree with your assessments.
        Autism is complex. There are strategies to avoid autism and there are strategies to minimise the effects. Minimising bBCM7 seems highly relevant to both.
        Human BCM7 is present mainly in early breastmilk (first few days) and unlikely to be important after the first few weeks. It is believed that it is linked to creation of the mother-baby bond but there is no definitive proof of thus. Thereafter the key issue is avoidance of bBCM7. Bovine BCM9 from A2 milk has antioxidant properties. These are relevant for multiple health conditions but we have no direct evidence in regard to autism relating specifically to positive effects of BCM9. There is strong evidence that autistic children have higher levels of bBCM7 in the blood and sera than other children. This may link both to intestinal permeability and to inability to upregulate DPP4 production, with this being the key enzyme that breaks down bBCM7. There is like to be a genetic element to this. In other words, autism links to bBCM7 (and most likely gliadorphin from gluten) in combination with a genetic susceptibility. I have a paper in the final stages of review for a public health journal that touches on these issues.
        KeithW
        Keith W

  8. Arnab Guha says:

    What about BCM4 or BCM6 or BCM8? Does these get formed through bovine milk? If yes, what are their characteristics in terms of lethality or it has any special benefits?

    • Keith Woodford says:

      Arnab,
      BCM4, 6 and 8 are unlikely to be formed in significant quantities. We know this from the principles of protein biochemistry.
      However, bovine BCM9 is formed from A2 beta-casein but not from a1 beta-casein.
      Our current understanding is that bBCM9 is a beneficial bioactive. There is need for more research to understand the details of this
      Keith

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